Professor Elzbieta Wyroba, Ph. D., Dr. Sci.

Nencki Institute of Experimental Biology, The Polish Academy of the Sciences,
3 Pasteur Street, 02-093 Warsaw, Poland
Email: e.wyroba@nencki.gov.pl


Head of the Laboratory of Cell Membrane Physiology in Department of Cell Biology 1991 - 2013.
Head of the Laboratory of Electron Microscopy (Core Facility Lab) 1979-91, 1994 -


1. Wyroba E, P. Kwaśniak, K. Miller, K. Kobyłecki, M. Osińska (2016) Site-directed mutagenesis, in vivo electroporation and mass spectrometry in search for determinants of the subcellular targeting of Rab7b paralogue in the model eukaryote Paramecium octaurelia Eur. J. Histochem. 60,99-106
2. Sobierajska K, Wieczorek K, Ciszewski WM, Sacewicz-Hofman I, Wawro ME, Wiktorska M, Boncela J, Papiewska-Pajak I, Kwasniak P, Wyroba E, Cierniewski CS, Niewiarowska J. (2016) β-III tubulin modulates the behavior of Snail overexpressed during the epithelial-to-mesenchymal transition in colon cancer cells. Biochim Biophys Acta - Molecular Cell Research 1863(9):2221-33
3. Ehrlich H, M. Motylenko, P. .V. Sundareshwar, A. Ereskovsky, I. Zgłobicka, T. Noga, T. Płociński, M. V. Tsurkan, E. Wyroba, S. Suski, H. Bilski, M. Wysokowski, H. Stöcker, A. Makarova, D. Vyalikh, J. Walter, S. L. Molodtsov, V. V. Bazhenov, I. Petrenko, (2016) Multiphase Biomineralization: Enigmatic Invasive Siliceous Diatoms Produce Crystalline Calcite Advanced Functional Materials 26, 2503 – 2510: + Supporting Information DOI: 10.1002/adfm.201504891, 1 – 23.
4. Łabuz J,S.Samardakiewicz,P.Hermanowicz,E.Wyroba,M.Pilarska,H. Gabryś (2016) Blue light-dependent changes in loosely bound calcium in Arabidopsis mesophyll cells: an X-ray microanalysis study J.Exp.Botany 67(13):3953-64
5. Wyroba E, S.Suski, K.Miller, R.Bartosiewicz (2015) Biomedical and agricultural applications of energy dispersive X-ray spectroscopy in electron microscopy Cellular & Molecular Biology Letters 20(3) 488-509
6. Satir BH, Wyroba E, Liu L, Lethan M, Satir P, Christensen ST. (2015) Evolutionary implications of localization of the signaling scaffold protein Parafusin to both cilia and the nucleus. Cell Biol Int. 39(2):136-45. doi: 10.1002/cbin.10337. Epub 2014 Jul
7. Boncela J, Przygodzka P, Wyroba E, Papiewska-Pajak I, Cierniewski CS. (2013) Secretion of SerpinB2 from endothelial cells activated with inflammatory stimuli. Exp Cell Res 319:1213-1219
8. Osińska M, Wiejak J., Wypych E, Bilski H., Bartosiewicz R, Wyroba E. (2011) Distinct expression, localization and function of two Rab7 proteins encoded by paralogous genes in a free-living model eukaryote. Acta Biochim. Polon. 58:597-607
9. Liu L, Wyroba E, Satir BH (2011) RNAi knockdown of parafusin inhibits the secretory pathway. Eur. J.Cell Biol. 90: 844 – 853
10. Boncela J, Przygodzka P, Papiewska-Pajak I, Wyroba E, Osinska M, Cierniewski CS (2011) Plasminogen activator inhibitor type 1 interacts with α3 subunit of proteasome and modulates its activity. J. Biol. Chem 286: 6820-6831
11. Boncela J, Przygodzka P, Papiewska-Pajak I, Wyroba E, Cierniewski CS. (2011) Association of plasminogen activator inhibitor type 2 (PAI-2) with proteasome within endothelial cells activated with inflammatory stimuli J Biol Chem. 286:43164-43171
12. Mackiewicz P, Wyroba E (2009) Phylogeny and evolution of Rab7 and Rab9 proteins. BMC Evolutionary Biology 9:101
13. Bednarek R, Boncela J, Smolarczyk K, Cierniewska-Cieslak A , Wyroba E, Cierniewski CS (2008) KU80 as a novel receptor for thymosin beta4 that mediates its intracellular activity different from G actin sequesterin J. Biol. Chem. 283:1534-44
14. Skalska J, Piwońska M, Wyroba E, Surmacz L, Wieczorek R, Koszela-Piotrowska I, Zielińska J, Bednarczyk P, Dołowy K, Wilczynski GM, Szewczyk A, Kunz WS. (2008) A novel potassium channel in skeletal muscle mitochondria. Biochim. Biophys. Acta 1777: 651–659
15. Wiejak J, Surmacz L, Wyroba E (2007) Pharmacological Attenuation of Paramecium Fluid-Phase Endocytosis. Folia biologica (Kraków) 55, 95-100
16. Wyroba E, Surmacz L, Osinska M, Wiejak (2007) Phagosome maturation in unicellular eukaryote Paramecium: the presence of RILP, Rab7 and LAMP-2 homologues. Eur J Histochem 51:163-172
17. Surmacz L, Wiejak J.,Wyroba E. (2006) Cloning of two genes encoding Acta Biochim. Polon. 53,149-56
18. Boncela J, Smolarczyk K, Wyroba E, Cierniewski CS. (2006) Binding of PAI-1 to endothelial cells stimulated by thymosin beta 4 and modulation of their fibrinolytic potential. J Biol Chem. 281,1066-72
19. Wiejak J., Surmacz L., Wyroba E. (2004) Dynamin-association with agonist-mediated sequestration of beta-adrenergic receptor in single-cell eukaryote Paramecium. J. Exp. Biol. 207, 1625-1632
20. Wiejak J., Surmacz L., Wyroba E. (2004) Dynamin- and clathrin dependent endocytic pathway in unicellular eukaryote Paramecium. Biochem. Cell Biol. 82, 547-558
21. Surmacz L., Wiejak J., Wyroba E. (2003) Evolutionary conservancy of the endocytic machinery in the unicellular eukaryote Paramecium. Biol. Cell 95, 69-74
22. Wiejak J., Surmacz L., Wyroba E. (2003) Dynamin involvement in Paramecium phagocytosis. Eur. J. Protistol. 39, 416-422
23. Filipek A., Jastrzębska B., Nowotny M., Kwiatkowska K., Hetman M., Surmacz L., Wyroba E., Kuźnicki J. (2002) Ca2+-dependent translocation of calcyclin binding protein (CacyBP) in neurons and neuroblastoma NB-2a cells. J. Biol. Chem. 277, 21103-21109
24. Wiejak J., Wyroba E. (2002) Dynamin: characteristics, mechanism of action and function. Cell. Mol. Biol. Lett. 7, 1073-1080
25. Wiejak J., Surmacz L., Wyroba E. (2002) Immunoanalogue of vertebrate beta-adrenergic receptor in the unicellular eukaryote Paramecium. Histochem. J. 34, 51-56
26. Wiejak J., Surmacz L., Wyroba E. (2001) Effect of wortmannin and phorbol ester on Paramecium fluid-phase uptake in the presence of transferrin. Eur. J. Histochem 45, 383-388
27. Surmacz L., Wiejak J., Wyroba E. (2001) Alteration in the protein pattern of subcellular fractions isolated from Paramecium cells supressed in phagocytosis. Folia Histochem. Cytochem 39, 301-305
28. Wyroba E., Satir B. (2000) Comparative hybridization analysis of yeast DNA with Paramecium parafusin - and different phosphoglucomutase– specific probes. Biochem. Cell Biol. 78, 683-690
29. Płatek A., Wiejak J., Wyroba E. (1999) RT-PCR and Northern blot analysis in search for putative Paramecium beta-adrenergic receptor. Acta Biochim. Polon 46, 1-9
30. Wyroba E., Płatek A. (1999) Modulation of endocytotic activity of Paramecium by the β-adrenergic ligands. Acta Protozool 38, 5-14.
31. Wiejak J., Płatek A., Surmacz L., Wyroba E. (1998) PCR amplification of Paramecium DNA using the β-adrenergic-specific primers Acta Protozool 37, 215-219
32. Surmacz L., Krawczyk K., Wyroba E. (1997) Different β-adrenergic-specific molecular probes reveal the same DNA species in Paramecium hybridization analysis. Cell. Mol. Biol. Lett. 2, 379-387
33. Wyroba E., Pawłowska Z., Kobylanska A., Pluskota E., Maszewska M., Stec W.J., Cierniewski C.S. (1996) Internalization of oligodeoxynucleotides antisense to type-1 plasminogen activator inhibitor mRNA in endothelial cells - a three-dimensional reconstruction by confocal microscopy. Biol. Cell 87, 37-44
34. Wyroba E., Widding Hoyer A., Storgaard P.S., Satir B.H. (1995) Mammalian homologue of the calcium-sensitive phosphoglycoprotein, parafusin. Eur. J. Cell Biol. 68, 419-426
35. Subramanian S.V., Wyroba E., Andersen A.P., Satir B.H. (1994) Cloning and sequencing of parafusin, a calcium-dependent exocytosis-related phosphoglycoprotein. Proc. Natl. Acad. Sci. USA 91, 9832-9836
36. Andersen A.P., Wyroba E., Zhao H., Reichman M., Satir B.H. (1994) The activity of parafusin is distinct from that of phosphoglucomutase in the unicellular eukaryote Paramecium. Biochem. Biophys. Res. Comm. 200, 1353-1358
37. Ucieklak A., Peche J., Łopatowska A., Wyroba E. (1993) Effect of propranolol on the duration of the reversal response in Paramecium octaurelia induced by KCl and BaCl. Acta Protozool 32, 27-32
38. Croce A.C., Wyroba E., Bottiroli G. (1992) Distribution and retention of rose bengal and disulphonated aluminiumphtalocyanine: a comparative study in unicellular eukaryote. J. Photochem Photobiol. B 16, 319-330
39. Croce A.C., Wyroba E., Cuzzoni C., Bottiroli G. (1992) Localization and persistance of rose bengal in unicellular eukariote and in experimental tumor. Excerpta Medica International Congress Series (Elsevier Science Publishers B.V., Netherlands) 737-741
40. Wyroba E. (1991) Quantitation of fluid phase uptake in Paramecium. 1. Kinetics in the cells blocked in phagocytic activity. Cell Biol. Int. Rep. 15, 1207-1216.
41. Croce A.C., Wyroba E., Bottiroli G. (1990) Uptake and distribution of hematoporphyrin-derivative in the unicellular eukaryote Paramecium. J. Photochem. Photobiol. B. 6, 405-417
42. Stoitsova S., Wyroba E. (1989) Structural variations of the surface coat along the strobila of Hymenolepis fraterna (Cestoda). Acta Parasitol. Pol. 34, 155-160
43. Wyroba E. (1989) Pharmacological specificity of beta-blockers induced inhibition of Paramecium phagocytosis. Acta Protozool. 28, 127-136
44. Wyroba E. (1989) Beta-adrenergic stimulation of phagocytosis in the unicellular eukaryote Paramecium aurelia. Cell Biol. Int. Rep. 13, 667- 678
45. Wyroba E. (1988) Endocytosis of cationized ferritin in Paramecium cells. Inst. Phys. Conf. Ser. No. 93. vol.3, 481-482.
46. Wyroba E., Bottiroli G., Giordano P.A. (1987) Membrane region of increased hydrophobicity in dividing Paramecium cells revealed by cycloheptaamylose-dansyl chloride complex. Eur. J. Cell Biol. 44, 34-38
47. Wyroba E. (1987) Stimulation of Paramecium phagocytosis by phorbol ester and forskolin. Cell Biol. Int. Rep. 11, 657-664
48. Cierniewski C.S., Janiak A., Wyroba E. (1986) Modification of fibrin network ultrastructure by Fab fragments specific to different domains of fibrinogen. Acta Biochim. Pol. 33, 195-202
49. Wyroba E. (1986) Effect of β-receptor antagonist dichloroisoproterenol on Paramecium endocytosis. Acta Protozool. 25, 167-174.
50. Giordano P., Wyroba E., Bottiroli G. (1985) Internalization of cycloheptaamylose-dansyl chloride complex during labelling of surface membrane in living Paramecium cells. Basic Appl. Histochem 29, 121-133
51. Wyroba E. (1984) Induced formation of abnormal cells in axenic culture of Paramecium aurelia. I. Cell surface and nuclear apparatus visualization using double sequential fluorescent labelling. Arch. Protistenk 128, 69-76
52. Wyroba E. (1984) Endocytosis of ruthenium red during staining of cell surface. In: Csanady A., Rohlick P., Szabo D. Electron Microscopy. Petofi Nyomada Kecskeme vol. 3, 1851-1852.
53. Wyroba E., Bottiroli G., Giordano P. (1983) Enhanced membrane fluorescence of CDC-labelled Paramecium subsequent to removal of surface components. Histochemistry 77, 171-175
54. Wyroba E., Bottiroli G., Giordano P. (1981) Autofluorescence of axenically cultivated Paramecium aurelia. Acta Protozool 20, 165-170
55. Wyroba E., Bottiroli G., Giordano P. (1981) Cycloheptaamylose-dansyl chloride complex as a fluorescent label of surface membranes in living ciliates. Histochemistry 73, 459-467
56. Wyroba E (1981) Alveolar system of Paramecium. I. Trapping of polycationic dye as a result of membrane impairment. Acta Histochem 69, 132-148
57. Wyroba E (1980) Release of Paramecium immobilization antigen to the non-nutrient medium. Cell Biol. Int. Rep. 4, 1-10
58. Wyroba E. (1980) Ultrastructural effects of hyaluronidase and phospholipase C on the pellicle of Paramecium aurelia. In: Electron Microscopy. Leiden vol. 2, 210-211
59. Wyroba E. (1978) Cell multiplication following partial enzymatic removal of surface coat. Cytobiologie 17, 412-420
60. Wyroba E., Brutkowska M. (1978) Restoration of phagocytic activity of Paramecium tetraurelia suppressed by previous digestion of surface coat. Acta Protozool 17, 515-524
61. Wyroba E. (1977) Studies on the surface coat of Paramecium aurelia. II. Relationship to the immobilization antigen Cell Tissue Res 181, 245- 253.
62. Przełęcka A., Wyroba E. (1974) Cytochemical studies on the surface coat of Paramecium aurelia. In: Electron Microscopy and Cytochemistry. Eds. Wisse E., et al., North Holland Publ. Co. Amsterdam 309-312
63. Wyroba E., Przełęcka A. (1973) Studies on the surface coat of Paramecium aurelia. I. Ruthenium red staining and enzyme treatment. Z. Zellforsch. 143, 343-353